Don't get me wrong. I really am making the effort to be productive. I put in extra hours to make up for the trillions of doctors appointments I must attend and I try to keep my schedule at work so that I don't waste too much time while I am here. You know, the whole work smarter concept.
The problem is, I'm not smarter. I feel like my brain is on strike and I make a lot of really silly mistakes that I normally do not make. Let's take yesterday for example:
- While performing a plasmid prep, I dumped all my samples into the waste container instead of onto the filter (which is where they needed to go).
- I forgot to heat shock half my transformations. I went straight from ice to the plate.
- I inoculated all of my overnight cultures into media containing antibiotics. Unfortunately, none of my strains were resistant to that antibiotic. In fact, these strains aren't resistant to any antibiotics.
So, yeah. Now I am even more behind. The cloning is already going very slowly thanks to the fact that E. coli fucking hates the constructs I am making. I can't really control this, but I'm doing what I can to minimize the problem. However, the things I can control, like say, using the correct antibiotic, I keep fucking up as well, making a process that is already going way too slow, move at a glacial pace.