Why is it that every damn time I try to leave the lab early on the Friday before a three day weekend, something always goes wrong?
The only major thing I was planning today was to run a couple of proteins through the Q column. Easy enough, right? Wrong.
The person who used the machine before me decided to fuck up every single dial on the data recorder as well as some unknown setting (unknown to me) on the actual machine. So, I ended up with fractions that were not the volume I set them to be, so now my protein is spread across a trillion tubes and I really don't know which ones because the graph I got back is completely unintelligible.
I was able to fix the data recorder before I started by second protein and now it is functioning properly, but I have no idea why the hell I am getting fractions that aren't at the volume I specified. Ugh.
I guess the ye olde science gods are punishing me for trying to get out of the lab without putting in the requisite minimum of 50 hrs.
The worst part is that everything went perfectly all week long. ugh.