tag:blogger.com,1999:blog-2697775263496385011.post5989769783827592396..comments2023-08-18T03:15:05.539-07:00Comments on Microbiologist XX: Damn these sequences to hell I saymicrobiologist xxhttp://www.blogger.com/profile/07578078880223116616noreply@blogger.comBlogger8125tag:blogger.com,1999:blog-2697775263496385011.post-40044851482547740012009-06-11T15:35:44.801-07:002009-06-11T15:35:44.801-07:00Dimitris - Excellent suggestion except that the pr...Dimitris - Excellent suggestion except that the primers were their own. Granted, that doesn't mean they know anything about them since as you said, these can be assembly line-like situations. :) <br />I think I'll give them one more chance, but only because I can leave my things that need sequencing at the front desk and they will pick them up.microbiologist xxhttps://www.blogger.com/profile/07578078880223116616noreply@blogger.comtag:blogger.com,1999:blog-2697775263496385011.post-63834893127036879642009-06-03T23:47:36.342-07:002009-06-03T23:47:36.342-07:00Hmmmm... Tell the people in that company your Tms ...Hmmmm... Tell the people in that company your Tms and GC content for the primers. They may use a std protocol ignoring any unique aspects of your vector/primers. People who do this are kind of assembly line workers; don't care much. Other option another company. We out here in LA use a local one LARAGEN and they're better than our core.Dimitrishttps://www.blogger.com/profile/06413415585468031896noreply@blogger.comtag:blogger.com,1999:blog-2697775263496385011.post-23765692481916341802009-06-02T07:38:19.936-07:002009-06-02T07:38:19.936-07:00TR - LOL! Ok, so there are worse things than shitt...TR - LOL! Ok, so there are worse things than shitty sequence.<br /><br />MH - Thanks for the tip!microbiologist xxhttps://www.blogger.com/profile/07578078880223116616noreply@blogger.comtag:blogger.com,1999:blog-2697775263496385011.post-33779328418486512312009-05-30T21:20:38.558-07:002009-05-30T21:20:38.558-07:00We've used Mass Gen's sequencing services before a...We've used Mass Gen's sequencing services before and been very happy with it. They're fast and relatively cheap. Good luck!Mad Hatterhttps://www.blogger.com/profile/12848061207872125237noreply@blogger.comtag:blogger.com,1999:blog-2697775263496385011.post-86687081477069091902009-05-29T22:06:19.259-07:002009-05-29T22:06:19.259-07:00You could always stick to animal work... =)You could always stick to animal work... =)Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-2697775263496385011.post-84749217387525109572009-05-29T12:58:13.276-07:002009-05-29T12:58:13.276-07:00The vector is pQE30 (N-terminal His tag, IPTG indu...The vector is pQE30 (N-terminal His tag, IPTG inducible promoter).<br />I don't see any homopolymeric tracts in the sequence. <br /><br />I am just now getting back to it :) I'll probably be ready to explode again in about 15m.microbiologist xxhttps://www.blogger.com/profile/07578078880223116616noreply@blogger.comtag:blogger.com,1999:blog-2697775263496385011.post-86648057462355870182009-05-29T10:27:19.685-07:002009-05-29T10:27:19.685-07:00Two questions: what vector aare you using, and do ...Two questions: what vector aare you using, and do you have any homopolymeric tracts towards the beginning of your sequence in the reverse stretch?Tomhttps://www.blogger.com/profile/14211618861743447072noreply@blogger.comtag:blogger.com,1999:blog-2697775263496385011.post-15904728365925647132009-05-29T10:21:43.974-07:002009-05-29T10:21:43.974-07:00MCLABMCLABTomhttps://www.blogger.com/profile/14211618861743447072noreply@blogger.com